RNA Sequencing

Overview

Enables transcriptome profiling, sophisticated gene expression analysis and discovery of new species of RNA.

The transcriptome is comprised of different populations of RNA molecules, including mRNA, rRNA, tRNA, and other non-coding RNA (such as microRNA, lncRNA). RNA sequencing (RNA-seq) using next-generation sequencing (NGS) technology allows us to profile the entire transcriptome, including both the coding and the noncoding regions. It also aids in the identification of genes that are differentially expressed in distinct cell populations and provides information on their relative abundance. In addition, it allows us to determine the effects of genetic variant splicing events, identify novel transcripts, detect gene fusions, isoforms and other structural variants, and call single nucleotide variants (SNVs).  

 

Why RNA Sequencing?

  • Enables the evaluation of RNA expression levels, variant splicing events, single nucleotide polymorphisms (SNPs), and insertion deletion (indels) events throughout the transcriptome
  • Provides the flexibility to evaluate specific populations of RNA through different technologies (targeted RNA sequencing, enrichment of mRNA or depletion of rRNA)
  • Facilitates the detection of low-abundance transcripts and isoforms through targeted sequencing
Roche Sequencing Solutions offers products for different parts of this workflow
Workflow Step
Product
Features and Benefits
cDNA Library Preparation

No RNA Enrichment

Standard

KAPA Stranded RNA-Seq Kits

Streamlined

KAPA RNA HyperPrep Kits

mRNA Capture

Standard

KAPA Stranded mRNA-Seq Kits

Streamlined

KAPA mRNA HyperPrep Kits

Ribosomal Depletion

Standard

KAPA Stranded RNA-Seq Kits with RiboErase

Streamlined

KAPA RNA HyperPrep Kits with RiboErase

  • RNA-seq or whole transcriptome
  • Traditional or streamlined workflow
  • mRNA-seq
  • Traditional or streamlined workflow
  • Non-coding RNA, whole transcriptome
  • Traditional or streamlined workflow 
Library Amplification
KAPA Library Amplification Kit
  • Minimization of PCR-induced bias of AT- and GC-rich library molecules for improved sequencing coverage of RNA
Library Quantification
KAPA Library Quantification Kits
  • Accurate quantification of adapter-ligated molecules prior to sample pooling and cluster generation for optimal sequencing results