Optimizing NGS sample preparation steps for automation
Automation of next-generation sequencing (NGS) sample preparation processes, such as library construction, quantification and normalization/pooling offers many advantages, including higher sample throughput, less hands-on time, greater reproducibility, and improved process control.
Roche Sequencing Solutions offers several kits and protocols optimized for automation. The KAPA DNA and RNA Library Preparation Kits are optimized for automated, high-throughput NGS library construction. In addition to automation-friendly protocols, 96-reaction library preparation kits contain the necessary overages required by automated liquid handling platforms. Validated protocols for KAPA library construction on Illumina systems are also available for various liquid handling platforms and methods are continuously being developed for new products and platforms.
KAPA Library Quantification Kits provide accurate, reliable, and high-throughput quantification of NGS libraries prior to normalization and pooling for multiplexed capture and/or sequencing. The workflow (sample dilutions and qPCR setup) is easy to automate in either 96- or 384-well format. Validated methods are available for selected platforms.
Why choose automated KAPA Library Preparation Methods?
- Wide breadth of platforms – validated methods for major 3rd party NGS liquid handling platforms
- High-quality libraries – carefully optimized and validated methods to produce similar library yields, complexity and sequence coverage as libraries prepared manually with our chemistry
- Flexibility – methods are compatible with a wide range of sample types and inputs, support different barcoding strategies, fragment sizes, batch sizes, and offer control over key library construction parameters for different workflows
- Convenience – minimal user intervention is required for batch processing of up to 96 samples