NGS Automation

Overview

Optimizing NGS sample preparation steps for automation

Automation of next-generation sequencing (NGS) sample preparation processes, such as library construction, quantification and normalization/pooling offers many advantages, including higher sample throughput, less hands-on time, greater reproducibility, and improved process control.

Roche Sequencing Solutions offers several kits and protocols optimized for automation. The KAPA DNA and RNA Library Preparation Kits are optimized for automated, high-throughput NGS library construction. In addition to automation-friendly protocols, 96-reaction library preparation kits contain the necessary overages required by automated liquid handling platforms. Validated protocols for KAPA library construction on Illumina systems are also available for various liquid handling platforms and methods are continuously being developed for new products and platforms.

KAPA Library Quantification Kits provide accurate, reliable, and high-throughput quantification of NGS libraries prior to normalization and pooling for multiplexed capture and/or sequencing. The workflow (sample dilutions and qPCR setup) is easy to automate in either 96- or 384-well format. Validated methods are available for selected platforms.

 

Why choose automated KAPA Library Preparation Methods?

  • Wide breadth of platforms – validated  methods for major 3rd party NGS liquid handling platforms
  • High-quality libraries – carefully optimized and validated methods to produce similar library yields, complexity and sequence coverage as libraries prepared manually with our chemistry
  • Flexibility – methods are compatible with a wide range of sample types and inputs, support different barcoding strategies, fragment sizes, batch sizes, and offer control over key library construction parameters for different workflows
  • Convenience – minimal user intervention is required for batch processing of up to 96 samples
Available products by instrument vendors

Instrument Vendor

Liquid Handling Platform

KAPA Kit

Sequencing Platform

Source

Status

PerkinElmer

Sciclone NGS and NGSx KAPA HTP/LTP Library Preparation Kit  

Illumina

Vendor

Available

    KAPA HyperPrep Library Preparation Kit

Illumina

Vendor

Available

    KAPA HyperPlus Library Preparation Kit

Illumina

Vendor

Available

    KAPA Stranded RNA-Seq Library Preparation Kit

Illumina

Vendor

Available

    KAPA Stranded mRNA-Seq Library Preparation Kit

Illumina

Vendor

Available

    KAPA Stranded RNA-Seq Library Preparation Kit with RiboErase

Illumina

Vendor

Available

   

KAPA Library Quantification Kit

Illumina,
Ion Torrent,
454, SoLiD

Vendor

Available

 

Zephyr NGS

KAPA HTP/LTP Library Preparation Kit

Illumina

Vendor

Available

    KAPA HyperPrep Library Preparation Kit

Illumina

Vendor

In Development

 

NGS Express

KAPA HTP/LTP Library Preparation Kit  

Illumina

Vendor

Available

    KAPA HyperPrep Preparation Kit

Illumina

Vendor

Available

   

KAPA Library Quantification Kit

Illumina,
Ion Torrent,
454, SOLiD

Vendor

Available

Beckman Coulter

Biomek FXp and FX dual-pod

KAPA HTP/LTP Library Preparation Kit

Illumina

Kapa

Available

    KAPA HyperPrep Library Preparation Kit

Illumina

Kapa

Available

    KAPA HyperPlus Library Preparation Kit

Illumina

Kapa

Available

    KAPA Stranded RNA-Seq Library Preparation Kit

Illumina

Kapa

Available

    KAPA Stranded mRNA-Seq Library Preparation Kit

Illumina

Kapa

Available

    KAPA Stranded RNA-Seq Library Preparation Kit with RiboErase

Illumina

Kapa

Available

 

Biomek FXp dual-pod and NX Span-8

KAPA Library Quantification Kit

Illumina,
Ion Torrent,
454, SOLiD

Vendor

Available

Agilent Technologies

Bravo NGS workstation

KAPA HTP/LTP Library Preparation Kit

Illumina

Vendor

Available

    KAPA HyperPrep Library Preparation Kit

Illumina

Vendor

Available

    KAPA HyperPlus Library Preparation Kit

Illumina

Vendor

Available

    KAPA Stranded RNA-Seq Library Preparation Kit

Illumina

Vendor

In Development

    KAPA Stranded mRNA-Seq Library Preparation Kit

Illumina

Vendor

In Development

   

KAPA Stranded RNA-Seq Library Preparation Kit with RiboErase

Illumina

Vendor

In Development

Eppendorf

epMotion 5075 TMX

KAPA HTP/LTP Library Preparation Kit

Illumina

Vendor

Available

    KAPA HyperPrep Library Preparation Kit

Illumina

Vendor

In Development

    KAPA HyperPlus Library Preparation Kit

Illumina

Vendor

In Development

Hamilton

STAR NGS Workstation

KAPA HyperPrep Library Preparation Kit

Illumina

Vendor

In Development

    KAPA HyperPlus Library Preparation Kit

Illumina

Vendor

In Development

    KAPA Stranded mRNA-Seq Library Preparation Kit

Illumina

Vendor

In Development

   

KAPA Library Quantification Kit

Illumina,
Ion Torrent,
454, SOLiD

Vendor

In Development

Tecan

Freedom Evo NGS Workstation

KAPA HTP/LTP Library Preparation Kit

Illumina

Vendor

In development

    KAPA HyperPrep Library Preparation Kit

Illumina

Vendor

In development

   

KAPA Library Quantification Kit

Illumina

Vendor

In development

Wafergen

Apollo 324

KAPA HTP/LTP Library Preparation Kit

Illumina

Roche

Guidelines for the use of KAPA reagents with existing DNA library prep methods

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