Epigenetics

Webinar: Discover epigenomic variation with a flexible targeted bisulfite sequencing method


Applications

Epigenetics research focuses on the effect of biological or chemical influences that can change the expression of a gene. As genes are comprised of a particular DNA sequence, biological interactions which surround the DNA may influence how a gene functions, thus the prefix epi, which is greek for “above or outside”, is an apt name for this study of genetics. Researchers use epigenetics to understand everything from variations in expressed phenotypes, to X-chromosome inactivation, to uncovering transcription errors.

Why Targeted Bisulfite Sequencing

DNA methylation has been shown to play an important role in a wide variety of biological processes, including silencing of transposable elements, stem cell differentiation, embryonic development, genomic imprinting and inflammation. Alteration of methylation patterns has been identified in many diseases including cancer, diabetes, cardiovascular disease, inflamation and neurological disorders.

Researchers have been using a number of approaches for methylation assessment, utilizing next-generation sequencing or microarray technologies. However, current research tools either provide limited efficiency or introduce experimental biases for genome-wide or targeted applications. Roche introduced the SeqCap Epi Enrichment System to overcome these challenges and greatly increase efficiency for a broad range of research applications.

The benefits of the SeqCap Epi system include

  • Discover differential methylation efficiently. Target your epigenomic region of interest with the first custom enrichment solution for bisulfite sequencing using a capture technology to deliver massive time and cost savings over whole genome or fixed content sequencing approaches.
  • Reveal hidden epigenetic modifications. Innovative probe design and manufacture allows for the capture of both strands enabling the detection of complex or rare methylation events.
  • Experience outstanding performance. Inherent flexibility of the system and the optimized bisulfite-then-capture workflow offer exceptional resolution, sensitivity and sequencing sample capacity